Fig. 5

Co-culturing with MSC^Wnt5a enhances the migration and angiogenesis of endothelial cells. a The representative scratch images of endothelial cells at 0 h and 24 h are presented. b Wound healing percentage of endothelial cells with different treatment was shown. c The tube formation assay was conducted to detect endothelial cell angiogenesis, and Calcein AM fluorescent dye was used to make the tubular structure more visible. d Quantitative analysis found that co-culturing with MSC^Wnt5a significantly enhanced endothelial cells angiogenesis compared to the MSC^Vector co-culture group and LPS group. Results are expressed as mean ± SD, n = 3. **P < 0.01, ****P < 0.0001 compared to control group, ^####P < 0.0001 compared to LPS group