Application of metagenomic next-generation sequencing in the clinical diagnosis of infectious diseases after allo-HSCT: a single-center analysis

Table 3 The mNGS and CMTs results of seven patients with repeated testing

Patient no.	Interval testing time for mNGS (Day)	Samples	mNGS	No. of reads	CMT	Blood culture	Clinical diagnosis	Outcome
26	15	Blood	TTV	199	Negative	Negative		Survival
26	15	BALF	EBV TTV HHV-6B	2001 67 80	EBV-DNA	Negative	EBV	Survival
34	72	Blood	Klebsiella pneumoniae TTV	137 18	Negative	Neisseria flavescens	Bacteremia	Survival
34	72	Blood	Aspergillus terreus Aspergillus nidulans Aspergillus fumigatus CMV	316 309 212 16	Negative	Negative	Pulmonary fungal infection	Died
36	14	Blood	HHV-6B CMV	7 1	Negative	Negative	HHV-6B Viral rash	Survival
36	14	Blood	CMV	5	Negative	Negative		Survival
44	8	Blood	Klebsiella pneumoniae HHV-6B EBV	74 4 4	Negative	Negative	Bacteremia	Survival
44	8	Blood	HHV-6B EBV CMV	409 12 11	Negative	Staphylococcus epidermidis	Staphylococcus epidermidis bacteremia	Died
46	0	Cerebrospinal fluid	Listeria monocytogenes	247	HBV-DNA (Blood)	Negative	Listeria monocytogenes meningitis	Survival
46	0	Cerebrospinal fluid	Negative	Negative	HBV-DNA (Blood)	Negative
48	10	Blood	Prevotella oris CMV	9 9623	CMV-DNA	Negative	CMV Viremia	Survival
48	10	Blood	Pseudomonas aeruginosa CMV TTV EBV	27,780 112,298 5 4	Negative	Negative	Pseudomonas aeruginosa	Survival
49	0	Cerebrospinal fluid	HHV-6B	950	Negative	Negative	HHV-6B encephalitis	Survival
49	0	Cerebrospinal fluid	Negative	Negative	Negative	Negative

mNGS Metagenomic next-generation sequencing, CMT Conventional microbiological test, TTV Torque teno virus, EBV Epstein-Barr virus, HSV-1 Human alpha herpes virus 1, HPV-3 Human polyomavirus 3, HHV-7 Human betaherpe svirus 7, BALF Bronchoalveolar lavage fluid, CMV Cytomegalovirus, IP Idiopathic pneumonia, BOS Bronchiolitis obliterans syndrome

ISSN: 1471-2334