Isolation and characterization of lytic bacteriophages from various sources in Addis Ababa against antimicrobial-resistant diarrheagenic Escherichia coli strains and evaluation of their therapeutic potential

Table 2 PCR primers used in phage identifications

Gene	Family	Sub-family	Genus	Sequence (5’→3’)	Reference
MCP	Myoviridae	Tevenvirinae	T4-like	T4-fw: CCC TGC TGT TCC AGA TCG ANA ARG ARG C	[35]
		Tevenvirinae	T4-like	T4-rev: CTG CCT GGC GTA CTG GTC DAT RWA NAC
		Ounavirinae	FO1-like	FO1-fw: CGC CAT TGA AGA ACT GCG TRW RCA YAT GGA
		Ounavirinae	FO1-like	FO1-rev: GGC ATC ATA TAG GAA TGC GCY TCR AAR TC
MCP	Podoviridae	Autographivirinae	T7-like	T7-fw: GAC AAG CGG AAG GAC ATC AAN CAY ACN GAR A
MCP	Podoviridae	Autographivirinae	T7-like	T7-rev: CGC GTA GTT GGC GGC RTT NGG CAT NA
McoP	Siphoviridae	-	T5-like	MCF-2F: GCGTGATGGTTGGGATGGTA	[36]
McoP	Siphoviridae	-	T5-like	MCF-2R: GACGCTCAATCTGACGACCA	[36]

ISSN: 1471-2334