Table 1 Escherichia coli pathotypes genes analyzed, and the primers used
Genes | Primer sequence (5’-3’) | Product size Bp | Anneling temp |
|---|---|---|---|
bfpA | F: AATGGTGCTTGCGCTTGCTGC | 324 | 62 |
R: CCGCTTTATCCAACCTGGTA | |||
stx1 | F: AGTTAATGTGGTGGCGAA | 817 | 58 |
R:GACTTGCCGCTTCCAT | |||
aggR | F: GTATACACAAAGAAGGAAGC | 254 | 58 |
R: ACAGAATCGTCAGCATCAGC | |||
LT | F: GCACACGGAGCTCCTCAGTC | 218 | 60 |
R: TCCTTCATCCTTTCAATGGCTTT | |||
sth | F: CCCTCAGGATGCTAAACCAG | 166 | 56 |
R: TTAATAGCACCCGGTACAAGC | |||
stp | F: TCTGTATTATCTTTCCCCTC | 186 | 65 |
R: ATAACATCCAGCACAGGC | |||
afa | F: GCTGGGCAGCAAACTGATAACT | 750 | 66 |
R: CATCAAGCTGTTTGTTCGTCCGC |