Table 1 Amplification and sequencing primers
Name | Sequence (5′-3′) | HXB2 position | Amplified fragment length (bp) |
|---|---|---|---|
Round 1 PCR | |||
Upstream primer MAW | TTGGAAATGTGGAAAGGAAGGAC | 2028 ~ 2050 | 151,226 |
Downstream primer RT21 | CTGTATTTCTGCTATTAAGTCTTTTGATGGG | 3509 ~ 3539 | |
Round 2 PCR | |||
Upstream primer PRO- | CAGAGCCAACAGCCCCACCA | 2147 ~ 2166 | 11,811 |
Downstream primer RT4R | CTTCTGTATATCATTGACAGTCCAGCT | 3299 ~ 3327 | |
Forward sequencing primers | |||
PROS3 | GCCAACAGCCCCACCA | 2151 ~ 2166 | 1176 |
RTAS | CTCAGATTGGTTGCAC | 2524 ~ 2539 | 803 |
RT-B | CCTAGTATAAACAATGAGACAC | 2946 ~ 2967 | 381 |
Reverse sequencing primers | |||
PROC1S | GCTGGGTGTGGTATTCC | 2826 ~ 2842 | 695 |
RT4R | CTTCTGTATATCATTGACAGTCCAGCT | 3299 ~ 3327 | 1180 |