Table 1 Primer and specific probes for the detection of Coxiella burnetii and Brucella spp., Brucella abortus, and Brucella melitensis
Species | Target | Primer | Sequencing primers (5’→3’) | Size fragments (bp) | Reference |
|---|---|---|---|---|---|
C. burnetii | IS1111 | Forward | 5’-AAAACGGATAAAAAGAGTCTGTGGTT-3’ | 70 | [25] |
Reverse | 5’-CCACACAAGCGCGATTCAT-3’ | ||||
Probe | 5’-6-FAM-AAAGCACTCATTGAGCGCCGCG-TAMRA-3’ | ||||
Brucellaspp. | IS711 | Forward | 5’-GCTTGAAGCTTGCGGACAGT-3’ | 63 | [26] |
Reverse | 5’-GGCCTACCGCTGCGAAT-3’ | ||||
Probe | 5’-FAM-AAGCCAACACCCGGCCATTATGGT-TAMRA-3’ | ||||
B. melitensis | BMEII0466 | Forward | TCGCATCGGCAGTTTCAA | 67 | [27] |
Reverse | CCAGCTTTTGGCCTTTTCC | ||||
Probe | TEX-CCTCGGCATGGCCCGCAA-BHQ-2 | ||||
B. abortus | BruAb2_0168 | Forward | GCACACTCACCTTCCACAACAA | 81 | [27] |
Reverse | CCCCGTTCTGCACCAGACT | ||||
Probe | HEX-TGGAACGACCTTTGCAGGCGAGATC-BHQ-1 |