Development and validation of multiplex real-time PCR for simultaneous detection of six bacterial pathogens causing lower respiratory tract infections and antimicrobial resistance genes

Table 4 Frequency of common AMR genes detected in microbiologically-confirmed target bacteria either phenotypically resistant to 3rd/4th cephalosporins, carbapenem, macrolide and oxacillin

		Cephalosporin resistance		Carbapenem resistance		Macrolide resistance		Oxacillin resistance
	Pathogens	Resistance	AMR genes	Resistance	AMR genes	Resistance	AMR genes	Resistance	AMR genes
	(n)	(n)	(n)	(n)	(n)	(n)	(n)	(n)	(n)
Gram negative	A. baumannii (9)	7	TEM (7/7)	7	OXA-23 (7/7)
	K. pneumoniae (5)	4	TEM (3/4)	2	NDM (2/2)
	K. pneumoniae (5)	4	CTXM-1 (1/4)	2	OXA-48 (2/2)
	E. coli (2)	1	SHV (1/1)	1
	E. coli (2)	1	TEM (1/1)	1
	P. aeruginosa (9)	1	CTXM-9 (1/1)	1	OXA-23 (1/1)
Gram positive	*S. aureus (5)					5	ermB (3/5)	4	mecA (3/4)

^*EG-mPCR detected 3 S. aureus and did not identify 2 S. aureus in single-infection cases

ISSN: 1471-2334