Table 3 Performance of EG-mPCR assays compared with the conventional culture method
Bacterial pathogens | EG-mPCR | Conventional culture method | % Sensitivity (95% CI) | % Specificity (95% CI) | % PPV (95% CI) | % NPV (95% CI) | Kappa | |
|---|---|---|---|---|---|---|---|---|
Positive | Negative | |||||||
K. pneumoniae | positive | 10 | 1 | 100 (69.2, 100) | 97.5 (86.8, 99.9) | 90.9 (58.7, 99.8) | 100 (91.0, 100) | 0.94 |
negative | 0 | 39 | ||||||
A. baumannii | positive | 14 | 1 | 100 (76.8, 100) | 97.2 (85.5, 99.9) | 93.3 (68.1, 99.8) | 100 (90.0, 100) | 0.95 |
negative | 0 | 35 | ||||||
P. aeruginosa | positive | 18 | 4 | 100 (81.5, 100) | 87.5 (71.0, 96.5) | 81.8 (59.7, 94.8) | 100 (87.7, 100) | 0.8 |
negative | 0 | 28 | ||||||
E. coli | positive | 2 | 2 | 100 (15.8, 100) | 95.8 (85.7, 99.5) | 50 (6.8, 93.2) | 100 (92.3, 100) | 0.65 |
negative | 0 | 46 | ||||||
S. aureus | positive | 7 | 2 | 63.6 (30.8, 89.1) | 94.9 (82.7, 99.4) | 77.8 (40.0, 97.2) | 90.2 (76.9, 97.3) | 0.63 |
negative | 4 | 37 | ||||||
S. pneumoniae | positive | 0 | 6 | NA | NA | NA | NA | NA |
negative | 0 | 44 | ||||||