Table 2 Tm values of primers used for singleplex and multiplex real-time PCR assays

Pathogen detection assays

 A. baumannii-F

ompA

80.6 ± 0.2

80.75 ± 0.27

0.3

0.3

 A. baumannii-R

 P. aeruginosa-F

oatA

87.6 ± 0.3

87.7 ± 0.3

0.4

0.5

 P. aeruginosa-R

 K. pneumoniae-F

khe

84.2 ± 0.3

84.22 ± 0.4

0.3

0.3

 K. pneumoniae-R

 E. coli-F

yaiO

86.8 ± 0.1

86.95 ± 0.4

0.2

0.4

 E. coli-R

 S. aureus-F

sa442

79 ± 0.16

79.12 ± 0.3

0.2

0.3

 S. aureus-R

 S. pneumoniae-F

ply

84.49 ± 0.13

84.66 ± 0.3

0.1

0.4

 S. pneumoniae-R

AMR gene detection assays

 mecA-F

mecA

76.49 ± 0.14

76.44 ± 0.7

0.19

0.92

 mecA-R

 ermB-F

ermB

79.83 ± 0.19

79.77 ± 0.73

0.24

0.91

 ermB-R

 OXA-48-F

bla_OXA-48

82.9 ± 0.08

81.98 ± 0.72

0.09

0.88

 OXA-48-R

 CTX-M-9-F

bla_CTX-M-9

84.72 ± 0.1

84.66 ± 0.69

0.12

0.81

 CTX-M-9-R

 SHV-F

bla_SHV

88.27 ± 0.1

87.85 ± 0.49

0.11

0.55

 SHV-R

 OXA-23-F

bla_OXA-23

78.26 ± 0.11

77.98 ± 0.39

0.14

0.51

 OXA-23-R

 mcr-1-F

mcr-1

81.8 ± 0.13

81.43 ± 0.55

0.16

0.67

 mcr-1-R

 TEM-F

bla_TEM

83.77 ± 0.16

83.41 ± 0.55

0.19

0.66

 TEM-R

 NDM-F

bla_NDM

87.22 ± 0.11

86.87 ± 0.54

0.12

0.62

 NDM-R

 mphA-F

mphA

89.43 ± 0.19

89.12 ± 0.46

0.22

0.51

 mphA-R

 IMP-F

bla_IMP

78.14 ± 0.15

78.68 ± 0.55

0.2

0.7

 IMP-R

 VanA-F

VanA

82.21 ± 0.22

82.61 ± 0.42

0.26

0.51

 VanA-R

 CTX-M-1-F

bla_CTX-M-1

85.69 ± 0.04

86.15 ± 0.57

0.04

0.66

 CTX-M-1-R

 KPC-F

bla_KPC

88.78 ± 0.5

89.4 ± 0.63

0.57

0.7

 KPC-R