Table 1 Sequences of primers used for singleplex and multiplex real-time EG-PCR assays
Primer | Gene | Sequence (5'-3') | Final conc.(μM) | Amplicon size (bp) | Reference |
|---|---|---|---|---|---|
Pathogen detection assays | |||||
 A. baumannii-F | ompA | GTTAAAGGCGACGTAGACG | 1 | 194 | this study |
 A. baumannii-R | TCAGAAGTACCACGTGTACCA | ||||
 P. aeruginosa-F | oatA | GAACTGCTCTTCCACCGACA | 0.6 | 170 | this study |
 P. aeruginosa-R | GCTGGTTCCTGATGGAGCC | ||||
 K. pneumoniae-F | khe | AGGGTTCGAGTTTGCTGCT | 1 | 171 | this study |
 K. pneumoniae-R | AGACAACCGCGTAGGCATT | ||||
 E. coli-F | yaiO | TGATTTCCGTGCGTCTGAATG | 1 | 115 | [20] |
 E. coli-R | ATGCTGCCGTAGCGTGTTTC | ||||
 S. aureus-F | sa442 | TCGGTACACGATATTCTTCAC | 1.2 | 179 | [18] |
 S. aureus-R | ACTCTCGTATGACCAGCTTC | ||||
 S. pneumoniae-F | ply | GAATTCCCTGTCTTTTCAAAGTC | 0.6 | 106 | this study |
 S. pneumoniae-R | GACCTTGTTAGAGAATAATCCCA | ||||
AMR gene detection assays | |||||
 mecA-F | mecA | GGCATCGTTCCAAAGAATGT | 0.8 | 128 | this study |
 mecA-R | AGTGGAACGAAGGTATCATCTT | ||||
 ermB-F | ermB | GTCTCGATTCAGCAATTG | 1.2 | 171 | this study |
 ermB-R | CAGTTGACGATATTCTCGAT |  | |||
 OXA-48-F | blaOXA-48 | GCGTGTATTAGCCTTATCGG | 0.5 | 204 | this study |
 OXA-48-R | GCGGGTAAAAATGCTTGGT | ||||
 CTX-M-9-F | blaCTX-M-9 | GTGCTTTATCGCGGTGA | 0.8 | 128 | this study |
 CTX-M-9-R | GCAGGCTTGATCTCGACAGG | ||||
 SHV-F | blaSHV | GGTGGATGCCGGTGACGA | 0.5 | 96 | this study |
 SHV-R | TCGGCAAGGTGTTTTTCGC | ||||
 OXA-23-F | blaOXA-23 | ACTTGCTATGTGGTTGCTTC | 0.8 | 106 | this study |
 OXA-23-R | GAATCACCTGATTATGTCCTT | ||||
 mcr-1-F | mcr-1 | TCGTATCGCTATGTGCTAAAGC | 0.5 | 128 | this study |
 mcr-1-R | TCGGTCTGTAGGGCATTTTG | ||||
 TEM-F | blaTEM | CCCTTTTTTGCGGCATTTTGC | 0.5 | 163 | this study |
 TEM-R | TTGGAAAACGTTCTTCGGGG |  | |||
 NDM-F | blaNDM | GACAATATCACCGTTGGGAT | 0.5 | 113 | this study |
 NDM-R | TAGTGCTCAGTGTCGGCAT | ||||
 mphA-F | mphA | AACTGTACGCACTTGCAG | 0.5 | 179 | this study |
 mphA-R | CAGCACCCGCGCCTCTGGTT | ||||
 IMP-F | blaIMP | GGTTTAAYAAAACAACCACC | 0.5 | 189 | this study |
 IMP-R | GGAATAGAGTGGCTTAAYTCTC | ||||
 VanA-F | VanA | TGTTTGGGGGTTGCTCAGA | 0.5 | 190 | this study |
 VanA-R | TATCCGGCGAGAGTACTGC | ||||
 CTX-M-1-F | blaCTX-M-1 | ACCACCAACGATATCGCGG | 0.5 | 143 | this study |
 CTX-M-1-R | TACAAACCGTCGGTGACGAT | ||||
 KPC-F | blaKPC | TCGTCGCGGAACCATTC | 0.8 | 121 | this study |
 KPC-R | ACAGTGGGAAGCGCTCCTC | ||||