Table 1 After sequential rounds of screening using the universal library sdAb-U and the targeted library sdAb-RBD, the SARS-CoV-2 RBD-specific binding phages were selectively enriched. PFU, plaque-forming unit
Library | Round | Wash times | Input titer (PFU/well) | Output titer (PFU/well) | Recovery efficiency | Fold increase |
|---|---|---|---|---|---|---|
sdAb-U | Round 1 | 25 | 3.2 × 1011 | 1.2 × 106 | 3.8 × 10− 6 | -- |
Round 2 | 30 | 2.1 × 1010 | 7.1 × 106 | 3.4 × 10− 4 | 89 | |
sdAb-RBD | Round 1 | 25 | 2.5 × 1010 | 3.9 × 106 | 1.6 × 10− 4 | 42 (to sdAb-U round 1) |
Round 2 | 30 | 5.1 × 109 | 1.0 × 106 | 2.0 × 10− 3 | 13 |