Fig. 2From: Single-domain antibodies against SARS-CoV-2 RBD from a two-stage phage screening of universal and focused synthetic librariesInitial screening of anti-RBD sdAbs. (A-B) Expression and purification of the recombinant RBD through baculovirus expression system and purification by Ni–NTA followed by gel filtration. C. The sequences of the CDR1 and CDR2 of the five high-affinity RBD-binding sdAbs. D. The results of phage ELISA of candidate phages. The error bars represent S.D. from three independent experiments. The statistical difference was measured by paired two-sided Student’s t-testBack to article page