Fig. 5

Host PBMC gene expression and functional analysis. A Principal-component analysis of the 4,311 differentially expressed genes (DEGs) among the four clinical phenotypes. Ellipses represent 95% confidence intervals. Percentages along x and y axes show the degree of variance explained by each principal component. The samples are coloured by the clinical phenotype i.e. A = Asymptomatic, U = Uninfected, FU = Febrile malaria ensuing from uninfected individuals and FA = febrile malaria ensuing from asymptomatic individuals. B Heatmap of the 4,311 DEGs (rows) among the four clinical phenotypes (columns) split into 4 clusters using K-means clustering and labelled c1 – c4. C, D, E and F Barplots showing enriched Gene Ontology (GO) terms in clusters c1, c2, c3 and c4, respectively, from the heatmap in B