Table 1 Selection criteria applied to studies found in the systematic review of studies evaluating the diagnostic accuracy of MAT, PCR, and IgM ELISA, published global and between 1950–2022
Selection criteria |
|---|
1) Studies performed using human blood samples |
2) Observational and interventional studies among patients with fever history or suspected leptospirosis |
3) Article in English, Spanish or Portuguese |
4) Test of interest (MAT, PCR targeting the Lfb1 gene or IgM ELISA with the target antigen Hurstbridge) and at least one comparator test (MAT, PCR with any target gene or Culture) performed on the same samples |
5) Data for extraction of a 2 × 2 contingency table |
6) For studies for MAT accuracy evaluation, results of testing single acute samples presented separately from results of testing paired samples (i.e. acute and convalescent samples) |
7) For studies for MAT accuracy evaluation, threshold for single acute-phase samples in endemic settings ≥ 1:400 and in non-endemic setting ≥ 1:100 |