Fig. 6

In-depth characterization of the CD8⁺ T cell clusters and relationship between proportions of various subpopulations. (A) Dot plot showing the percentages of CD8⁺ T cell clusters across the four conditions. Significant differences are indicated by *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Differences between each group were analyzed using a two-sided unpaired Mann–Whitney U-test. (B) Bubble heatmap showing Spearman’s correlation of CD8⁺ T cell cluster percentages with viral reservoir parameters, CD4/CD8 ratio, and CD4⁺ T cell count. The color of the bubble represents the correlation coefficient, the deeper the more relevant between the two variables, while red and blue indicate a positive and negative correlation. respectively. The size of the bubble indicates statistically significant P-values, with larger values representing greater significance. (C) Heatmap showing Spearman correlations between the frequencies of selected cell subsets and clusters as indicated. Red and blue indicate positive and negative associations, respectively. The deeper the more relevant between the two variables.