Fig. 2
Characterization of myeloid cells from PBMCs of TNs, INRs, IRs and HCs. (A) A viSNE map of gated myeloid live single cells depicting the myeloid cell landscape. Different groups are colored as indicated (right). (B) Heatmap showing the median metal intensity of individual markers for each cluster as indicated. (C) Violin plots showing the expression of selected markers on global myeloid cells of HCs and patients with chronic HIV-1 infection. (D) Histograms showing expression of indicated markers for 11 myeloid cell subpopulations, including pDC, cDC1, cDC2, DN DC, non-classical monocytes (ncMono), detailed classical monocytes (cMono) according to CXCR3 expression, and intermediate monocytes (intMono). (E) Dot plot showing the frequencies of myeloid cell subsets from individuals with chronic HIV-1 infection and healthy individuals. Groups are shown in different colors. Horizontal lines represent mean values, and each dot represents one sample. Significant differences are indicated by *P < 0.05; **P < 0.01; ***P < 0.001. Differences between each group were analyzed using a two-sided unpaired Mann–Whitney U-test. (F) Bubble heatmap showing Spearman’s rank correlation of viral reservoir parameters, CD4/CD8 ratio, and CD4+ T cell count with myeloid cell subset percentages. The color of the bubble represents the correlation coefficient, the deeper the more relevant between the two variables, while the direction is indicated by colors: red for a positive correlation and blue for a negative correlation. The size of the bubble indicates statistically significant P-values, with larger values representing greater significance