Table 1 Primer sequences and their amplicon length to detect SA and MRSA in culture isolates
Gene region | Primer Sequence (5’- 3’) | Amplicon Sequence |
|---|---|---|
nuc gene | 5’ GCGATTGATGGTGATACGGT 3’ | 279 bp |
5’ AGCCAAGCCTTGACGAACTAAAGC 3’ | ||
mecA gene | 5’ AAAATCGATGGTAAAGGTTGGC 3’ | 533 bp |
5’ AGTTCTGCAGTACCGGATTTGC 3’ | ||
16S gene | 5’ GTTATTAGGGAAGAACATATGTG 3’ | 750 bp |
5’ CCACCTTCCTCCGGTTTGTCACC 3’ | ||
PVL gene | 5’ ATCATTAGGTAAAATGTCTGGACATGATCCA 3’ | 410 bp |
5’ GCATCAAGTGTATTGGATAGCAAAAGC 3’ |