Species identification and drug susceptibility testing of non-tuberculous mycobacteria by Line Probe Assay in Lambaréné, Gabon—a cross-sectional study

Table 4 Associated mutations and drug susceptibility results for macrolides and aminoglycosides in the GenoType1 Mycobacterium NTM-DR assay

Species/subspecies	Number of isolates	Gene						Macrolides	Aminoglycosides
		*erm* (41)		*rrl*		*rrs*
		C28	T28	WT	MUT	WT	MUT
M. intracellulare	53	NA	NA	53	0	53	0	S	S
M. avium	27	NA	NA	27	0	27	0	S	S
M. chelonae	17	NA	NA	17	0	17	0	S	S
M. abscessus subsp. massiliense	10	10	0	10	0	10	0	S	S
M. abscessus subsp. abscessus	15	15	0	15	0	15	0	S	S
M. abscessus subsp. abscessus	5	0	5	5	5	5	5	R	R
M. abscessus subsp. bolletii	8	8	0	8	0	8	0	S	S

erm (41) the erm (41) gene is examined for detection of resistance to macrolides (clarithromycin or azithromycin) and is only present in members of the M. abscessus complex
erm (41) C28 probe detects a genotype that carries a C at position 28 of the erm (41) gene. When the erm (41) C28 probe stains positive, this indicates that the tested strain is sensitive to macrolides (except for strains with an additional rrl mutation)
erm (41) T28 probe detects a genotype that carries a T instead of a C at position 28 of the erm (41) gene. When the erm (41) T28 probe stains positive, this indicates that the tested strain is resistant to macrolides
rrl, the rrl gene is examined for detection of resistance to macrolides (clarithromycin or azithromycin)
WT Wild type, MUT Mutation, S Susceptible, R Resistant and NA Not applicable

ISSN: 1471-2334