Fig. 1

(a) A thin blood film made from blood from the case of malaria and stained with Giemsa’s stain as seen microscopically at X1000 magnification. Red numbers indicate erythrocytes infected with immature trophozoites (ring form) of Plasmodium spp. Erythrocyte 3 shows the double chromatin configuration (headphones) and erythrocyte 4 is in the accolé (applique) position, both of which are indicative of an infection of Plasmodium spp.(b) An agarose gel showing the amplification of region rPLU6-rPLU5, the region of the 18S rRNA gene, showing: 1, the 100 bp marker; 2 and 3, two samples, one after one week and the other after two weeks post-treatment; 4, pre-treatment sample during the acute phase; 5 and 6, negative and positive controls, respectively