Table 2 The detection of clinical samples by CIRSPR-Cas13a lateral flow strip
From: Highly sensitive and rapid point-of-care testing for HIV-1 infection based on CRISPR-Cas13a system
RT-qPCR | Sensitivity | Specificity | PPV | NPV | Accuracy | ||
|---|---|---|---|---|---|---|---|
Positive | Negative | (95% CI) | (95% CI) | (95% CI) | (95% CI) | (95% CI) | |
CRISPR-Fluorescence | |||||||
Positive | 101 | 0 | 91.81% | 100% | 100% | 39.14% | 92.22% |
Negative | 9 | 48 | (85.03–96.19%) | (92.60–100%) | (96.41–100%) | (25.59–54.60%) | (86.89–95.88%) |
Total | 110 | 48 | |||||
CRISPR-strip | |||||||
Positive | 101 | 0 | 91.81% | 100% | 100% | 39.14% | 92.22% |
Negative | 9 | 48 | (85.03–96.19%) | (92.60–100%) | (96.41–100%) | (25.59–54.60%) | (86.89–95.88%) |
Total | 110 | 48 | |||||