Fig. 6
From: Highly sensitive and rapid point-of-care testing for HIV-1 infection based on CRISPR-Cas13a system
RT-RAA-CRISPR-Cas13a detected HIV-1 in 158 plasma clinical samples. Positive HIV-1 clinical samples were classified according to their Ct values from RT-qPCR and the total number of clinical samples in each Ct classification is shown. Representative lateral-flow strip and fluorescence detection of each Ct bin as well as HIV-1 negative samples are shown. The full dataset is shown in Supplementary Fig. 1. For lateral-flow strip readings, the disappearance of the colored test strip, often combined with the appearance of the colored control strip, indicates a positive HIV-1 result. For fluorescence readings, we set a threshold for the signal-to-noise (S/N) ratio of fluorescence intensity (blue line) (noise is the fluorescence intensity from negative samples performed in parallel with water as input), and positive results are 3. Bottom row shows the number of false negative samples; false negative result rates are shown for each Ct bin and for the two RT-RAA-CRISPR-Cas13a readout modes