Fig. 5
From: Highly sensitive and rapid point-of-care testing for HIV-1 infection based on CRISPR-Cas13a system
Evaluation of the specificity of CRISPR-Cas13a for HIV-1 detection. A The genome map shows the site of the target crRNA and primers on the HIV-1 pol gene. RT-RAA primers have degenerate bases R (A/G) and Y (C/T), which are marked in red; the crRNA sequence is marked in yellow. B Fluorescence method CRISPR-Cas13a detects 5 kinds of pathogen nucleic acids. C Fluorescence value of different pathogens when CRISPR-Cas13a is detected for 10 min by fluorescence method. D Test strip method CRISPR-Cas13a to detect nucleic acids of different pathogens. E Band intensities of each test strip were quantified using ImageJ. C: Control line, T: Test line, NC: Negative control, "****" means P < 0.0001, the above experiments were performed 3 independent repeat experiments