Fig. 4
From: Highly sensitive and rapid point-of-care testing for HIV-1 infection based on CRISPR-Cas13a system
Evaluation of the CRISPR-based latera-flow strip for HIV-1 RNA detection. A-B CRISPR-based fluorescence assay for detecting HIV-1 RNA concentration at 1 h. C-D CRISPR-based latera-flow strip assay for HIV-1 RNA detection and the band intensities. E–F Distribution of Ct values of RT-qPCR method and amplification curve of HIV-1 RNA at different concentrations. G Agarose gel electrophoresis of HIV-1 RNA with different concentrations after RT-PCR (The uncropped result is shown in Supplementary Figure S3). H Agarose gel electrophoresis of HIV-1 RNA with different concentrations after RT-RAA (The uncropped result is shown in Supplementary Figure S3). NC: Negative control, "****" means P < 0.0001, "***", "**" means P < 0.005, and "ns" means no significant difference. All experiments were carried out in triplicate for data analysis