Fig. 2
From: Highly sensitive and rapid point-of-care testing for HIV-1 infection based on CRISPR-Cas13a system
Screening of RT-RAA primers and crRNA. A The HIV-1 RNA template was diluted in gradient and amplified separately using 9 pairs of RT-RAA primers. The amplification products are shown in the red dashed boxes (The uncropped result is shown in Supplementary Figure S1). M refers to Marker, NC refers to ddH2O. B HIV-1 RNA templates (concentration of 1 × 105 copies/μL and 1 × 101 copies/μL) are used for RT-RAA amplification and detected by CRISPR-based fluorescence assay. M refers to Marker, NC refers to negative control