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Table 3 Intra-assay and inter-assay coefficients of variation for the pentaplex qRT-PCR

From: Development and clinical validation of a one-step pentaplex real-time reverse transcription PCR assay for detection of hepatitis virus B, C, E, Treponema pallidum, and a human housekeeping gene

Target(copies/µL)

Intra-assay CV(%)

Inter-assay CV(%)

HBV

HCV

HEV

T.pallidum

RNase P

HBV

HCV

HEV

T.pallidum

RNase P

106

1.69

0.68

1.60

1.13

1.74

1.66

1.05

1.72

1.93

2.00

105

1.19

0.57

1.17

0.94

1.35

1.63

0.68

1.84

1.57

1.48

104

1.08

0.42

0.93

0.78

0.60

1.67

0.80

1.52

1.23

1.10

103

0.29

0.18

1.47

0.50

0.85

1.58

1.20

1.66

0.89

1.17

102

0.75

0.17

0.86

0.73

0.59

0.83

0.69

1.01

1.13

0.62

101

0.44

0.33

1.30

0.64

1.61

0.66

0.98

1.74

1.81

1.61

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BMC Infectious Diseases

ISSN: 1471-2334

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