Fig. 2

Cross-streak assay with Pseudomonas aeruginosa and Staphylococcus aureus. Two different P. aeruginosa strains (0093, ATCC 27,853, horizontal) were cross-streaked with seven PVL- positive and seven PVL-negative S. aureus from this study (A-C). The growth of S. aureus was scored 0 points (full inhibition by P. aeruginosa, A), 1 point (interruption of the S. aureus streak at the crossing with the P. aeruginosa streak, B) and 2 points (no interruption of the S. aureus streak, C). The scores of three independent tests were added to calculate the S. aureus growth score (min. 0, max. 6). The growth scores were comparable between PVL-positive and PVL-negative S. aureus isolates (D). However, the growth was significantly dependent on the competitor P. aeruginosa strain (E)