Fig. 2

A Diagnostic accuracy of Siemens Enzygnost EIA kit compared to PRN reported in high quality studies. % sensitivity and specificity presented. CI, confidence interval. EIA, enzyme immunoassay. FN, false negatives. FP, false positives. NE, not estimable. NR, not reported. TN, true negatives. TP, true positives. *Study classified EIA equivocals as EIA negative. **Study excluded EIA equivocals. All other studies classified EIA equivocals as EIA positive. All studies used the Enzygnost EIA kit with a threshold of < 0.1 O.D (except Warrener where the threshold was not reported). All PRNT tests reported to used a threshold of ≥ 120 mIU/mL except Cohen 2008 which used a batch-specific thresholds and Tischer et al. which reported to use “40 ± 20mIU/mL”. We do not report any comparisons that used EIA thresholds from eg., 8mIU/mL. Cohen 2008 authors reported weighted estimates, unweighted estimates displayed. All papers tested samples by both index and reference tests except Cohen 2008 (both these all samples by PRNT and selected a subset of those samples for EIA testing).” B Diagnostic accuracy of non-Siemens Enzygnost EIA kits compared to PRN reported in high quality studies. % sensitivity and specificity presented. CI, confidence interval. EIA, enzyme immunoassay. FN, false negatives. FP, false positives. MBA, Multiplex bead assay. MIA, Multiplex immunoassay. MeV N, recombinant measles virus nucleoprotein. MeV WVAL, Laboratory-produced purified measles whole-virus antigen. MeV WVAc, Commercially produced whole-virus antigen. NE, not estimable. NR, not reported. TN, true negatives. TP, true positives. *Study classified EIA equivocals as EIA negative. **Study excluded EIA equivocals. ***How EIA equivocal were treated was not reported. All other studies classified EIA equivocals as EIA positive. 1)EIA threshold of < 40mIU/mL and PRNT threshold of ≥ 40mIU/mL 2) EIA threshold of < 100mIU/mL and PRNT threshold of ≥ 100mIU/mL. All samples were tested by both index and reference tests but the small number of PRN positive samples by EIA threshold of < 100mIU/mL and PRNT threshold of ≥ 100mIU/mL limited our ability to estimate sensitivity. All PRNT tests reported to used a threshold of ≥ 120 mIU/mL except Goncalves 1999 et al. (at birth age group) used a threshold of 40mIU/mL, Mao 2009 et al. used threshold 1:4 titer, Cohen 2006 et al. used a batch-specific threshold, Lee 1999 et al. used a threshold of > 200mIU/ml, Hatchette 2017 et al. used a threshold of > 192mIU/mL and deSouza 1991 et al. did not report a threshold. EIA equivocals were grouped differently depending on the study. All papers tested samples by index and reference tests except Fowlkes 2011(tested random subset of EIA tested samples by PRNT), Lee 1999 (both these all samples by PRNT and selected a subset of those samples for EIA testing). BioPlex 2200 MMRV IgG is reported as “BioPlex 2200””