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Table 6 Output from 1928 and INH where genotypic prediction of antibiotic susceptibility test showed VME (bold) or ME (underlined)

From: Benchmarking of two bioinformatic workflows for the analysis of whole-genome sequenced Staphylococcus aureus collected from patients with suspected sepsis

 

Disc diffusion

Phenotypic AST

Bioinformatic workflows

Genotypic predicted AST

Isolate

Antibiotic (R or S)

R or S

Resistance markers identified by 1928

R or S

Genes conferring antimicrobial resistance identified by INH

SA 907

Ciprofloxacin (R)

R

grlA (S80F + I45M)a

grlB (E422D)

S

No genes

SA 1525

Ciprofloxacin(R)

R

grlA (S80F)

S

No genes

SA 1852

Ciprofloxacin(R)

R

grlA (S80F)

S

No genes

SA 1153

Ciprofloxacin(R)

R

grlA (S80F)

gyrA (S84L)

S

No gene

SA 1046

Ciprofloxacin(S)

R

grlA (S80F + I45M)

grlB (E422D)

S

No genes

SA 61

Clindamycin(R)

S

No resistance markers

S

No genes

SA 63

Clindamycin(R)

S

No resistance markers

S

No genes

SA 804

Clindamycin(R)

S

No resistance markers

S

No genes

SA 804

Erythromycin(R)

S

No resistance markers

S

No genes

SA 535

Fusidic acid (R)

R

fusC

S

No genes

SA 846

Fusidic acid (R)

R

fusC

S

No genes

SA 1162

Fusidic acid (R)

R

fusC

S

No genes

SA 1333

Fusidic acid (R)

S

No resistance markers

S

No genes

SA 1349

Fusidic acid (R)

S

No resistance markers

S

No genes

SA 1640

Fusidic acid (R)

S

No resistance markers

S

No genes

SA 1370

Fusidic acid (R)

S

No resistance markers

S

No genes

SA 1197

Isoxazolyl penicillin (S)

R

mecC

R

mecA

  1. a () within brackets detected mutations are indicated. VME: Very major error: resistant phenotype predicted as a susceptible genotype. ME: Major error: susceptible phenotype predicted as resistant genotype. R-resistant; phenotypic AST R is retrieved from the EUCAST assay, genotypic AST R was conferred by the presence of resistance markers or genes, S-susceptible; phenotypic AST S was retrieved from the EUCAST assay, genotypic S was conferred by the absence of resistance markers and genes. INH-in-house pipeline using Resfinder