Fig. 3

Adaption of Borrelia multiplex to the INTELLIFLEX platform. Adaption of the Borrelia multiplex to the new INTELLIFLEX platform was done in three steps. A, B 4 serum samples were measured for Borrelia specific IgG (A) and IgM (B) antibodies to compare the PE detection system on FLEXMAP 3D and INTELLIFLEX platform. C 12 serum samples were measured for IgG antibodies to compare a directly PE-labelled detection antibody with a biotinylated antibody in combination with BV421 labelled streptavidin. D, E 12 serum samples were measured for IgG (D) and IgM (E) antibodies separately (single detection) or in combination (dual detection). In each graph MFI signals were plotted against each other and analyzed by linear regression. A linear curve (x = y) shown as red dashed line indicates identical MFI signals for detection systems. Correlation analysis was performed after Spearman