Fig. 2

Antibody response in Borrelia negative and positive samples. 341 serum samples from the MEMO study were measured with the Borrelia multiplex and a commercial bead-based immunoassay (recomBead Borrelia IgG/IgM 2.0). Based on the result of the recomBead Borrelia samples were categorized in Borrelia negative and positive. The corresponding MFI signals of the Borrelia multiplex were plotted in a Box-Whisker plot. Boxes include the median and the 25th and 75th percentiles. Whiskers are limited to 1.5 times IQR. Outliers are shown as depicted circles. A dashed line (S/CO = 1.0) indicates the determined MFI cut-offs. For the antigens DbpA–ZS7, DbpA–A14S and DbpA–PBi no cut-offs were needed. For statistical analysis Mann–Whitney test (two-tailed) was used. P values were classified as follows: > 0.05 (ns), ≤ 0.05 (*), ≤ 0.01 (**), ≤ 0.001 (***). A–F Box-Whisker plots for IgG dominant antigens. G, H Box-Whisker plots for IgM dominant antigens