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Fig. 3 | BMC Infectious Diseases

Fig. 3

From: Assessment of genotypes, endosymbionts and clinical characteristics of Acanthamoeba recovered from ocular infection

Fig. 3

Cropped agarose gel image of PCR amplicons of Acanthamoeba isolates and intracellular bacteria. Bands were visualised using 1% gel electrophoresis; primer pairs JDPFw/Rv and 515Fw/806Rv yielded ~ 450 bp and ~ 293 bp amplicons, respectively. A. castellanii (ATCC 30868) and E. coli (ATCC 10798) were used as positive control for 18S rRNA and 16S rRNA PCR reactions and nuclease free water was used as negative control. Full size gel images are included in Additional file 1 (Figs. 4 and 5)

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