Fig. 4

Simple flowchart of the mNGS process, highlighting common inconsistencies. ① Skin-derived microorganisms can be introduced during lumbar puncture, resulting in false positive mNGS results. ② The main distribution of different types of pathogens in the brain. ③ The supernatant was processed for further detection after centrifugation. ④ Common contaminants introduced when adding reagents for removing human hosts. ⑤ The choice of sequencing method also influenced the results. ⑥ Sequencing depth also affects the detection efficiency