Table 2 Assessment of minimal requirement reads for antiviral mutation detection
Gene | UL54 | UL97 | |
|---|---|---|---|
Mutation | V787L | K513N | M460V |
Gene position | 79,564 | 80,384 | 143,175 |
Reference | C | C | A |
Full Reads | Total count: 50,643 | Total count: 53,339 | Total count: 626,029 |
 A: 696 (1%) | A: 39,503 (74%) | A: 185,419 (30%) | |
C: 24,055 (47%) | C: 10,983 (21%) | C: 6005 (1%) | |
G: 24,500 (48%) | G: 2560 (5%) | G: 428,708 (68%) | |
T: 1392 (3%) | T: 293 (1%) | T: 5897 (1%) | |
DEL: 5075 | DEL: 2333 | DEL: 18,162 | |
INS: 1278 | INS: 1922 | INS: 11,573 | |
1000 Reads | Total count: 805 | Total count: 833 | Total count: 948 |
 A: 12 (1% ) | A: 596 (72%) | A: 291 (31%) | |
C: 393 (49%) | C: 198 (24%) | C: 7 (1%) | |
G: 382 (47%) | G: 37 (4%) | G: 642 (68%) | |
T: 18 (2%) | T: 2 (0%) | T: 8 (1%) | |
DEL: 76 | DEL: 48 | DEL: 21 | |
INS: 24 | INS: 27 | INS: 17 | |
30 Reads | Total count: 23 | Total count: 24 | Total count: 27 |
 A: 0 |  A: 17 (71%) | A: 10 (37%) | |
C: 14 (61%) | C: 5 (21%) | C: 0 | |
G: 9 (39%) | G: 2 (8%) | G: 17 (63%) | |
T: 0 | T: 0 | T: 0 | |
DEL: 2 | DEL: 1 | DEL: 0 | |
INS: 0 | INS: 2 | INS: 2 | |