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Table 2 Assessment of minimal requirement reads for antiviral mutation detection

From: Detection of antiviral drug resistance in patients with congenital cytomegalovirus infection using long-read sequencing: a retrospective observational study

Gene UL54 UL97
Mutation V787L K513N M460V
Gene position 79,564 80,384 143,175
Reference C C A
Full
Reads
Total count: 50,643 Total count: 53,339 Total count: 626,029
 A: 696 (1%) A: 39,503 (74%) A: 185,419 (30%)
C: 24,055 (47%) C: 10,983 (21%) C: 6005 (1%)
G: 24,500 (48%) G: 2560 (5%) G: 428,708 (68%)
T: 1392 (3%) T: 293 (1%) T: 5897 (1%)
DEL: 5075 DEL: 2333 DEL: 18,162
INS: 1278 INS: 1922 INS: 11,573
1000
Reads
Total count: 805 Total count: 833 Total count: 948
 A: 12 (1% ) A: 596 (72%) A: 291 (31%)
C: 393 (49%) C: 198 (24%) C: 7 (1%)
G: 382 (47%) G: 37 (4%) G: 642 (68%)
T: 18 (2%) T: 2 (0%) T: 8 (1%)
DEL: 76 DEL: 48 DEL: 21
INS: 24 INS: 27 INS: 17
30
Reads
Total count: 23 Total count: 24 Total count: 27
 A: 0  A: 17 (71%) A: 10 (37%)
C: 14 (61%) C: 5 (21%) C: 0
G: 9 (39%) G: 2 (8%) G: 17 (63%)
T: 0 T: 0 T: 0
DEL: 2 DEL: 1 DEL: 0
INS: 0 INS: 2 INS: 2
  1. Single nucleotide variant (SNV) mutation detectable by mutation resistance analyzer (bold)