Resistance to single dose albendazole and reinfection with intestinal helminths among children ages 2 to 11 years from the Peruvian Amazon region: a study protocol

Table 1 Primers used in multiplex qPCR analysis of stool samples by STH1 and STH2

qPCR	Target	Primer	Sequence (5′–3′)	Size (bp)	Gene detected	Final conc. (nM)
STH1	Strongyloides stercoralis	Strongy-F	GAATTCCAAGTAAACGTAAGTCATTAGC	101	18S	100
		Strongy-R	TGCCTCTGGATATTGCTCAGTTC			100
		Strongy-P	FAM-ACACACCGG/ZEN/CCGTCGCTGC-IBFQ			50
STH1	Trichuris trichiura	Tri-F	TTGAAACGACTTGCTCATCAACTT	76	18S	100
		Tri-R	CTGATTCTCCGTTAACCGTTGTC			100
		Tri-P	CY5-CGATGGTAC/TAO/GCTACGTGCTTACCATGG-IBRQ			50
STH1	Ascaris lumbricoides	Asc-F	GTAATAGCAGTCGGCGGTTTCTT	87	Internal transcribed spacer 1	100
		Asc-R	GCCCAACATGCCACCTATTC			100
		Asc-P	HEX-TTGGCGGAC/ZEN/AATTGCATGCGAT-IBFQ			50
STH2	Ancylostoma spp.	Anc-F	CGGGAAGGTTGGGAGTATC	104	Internal transcribed spacer 1	100
	Ancylostoma spp.	Anc-R	CGAACTTCGCACAGCAATC			100
	A. duodenale	Aduo-P	HEX-TCGTTAC + T + GGGTGACGG-IBFQ			50
	A. ceylanicum	Acey-P	FAM-CCGTTC + CTGGGTGGC-IBFQ			50
STH2	Necator americanus	Nec-F	CTGTTTGTCGAACGGTACTTGC	101	Internal transcribed spacer 2	100
		Nec-R	ATAACAGCGTGCACATGTTGC			100
		Nec-P	CY5-CTG+TA+CTA+CG+CAT+TGTATAC-IBRQ			50
STH1 Y STH 2	Phocine herpes virus	PhHV-F	GGGCGAATCACAGATTGAATC		gB gene	100
		PhHV-R	GCGGTTCCAAACGTACCAA			100
		PhHV-P	Cy5-TTTTTATGTGTCCGCCACCATCTGGATC-BHQ2			50

ISSN: 1471-2334