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Fig. 3 | BMC Infectious Diseases

Fig. 3

From: Enhanced serodiagnosis of melioidosis by indirect ELISA using the chimeric protein rGroEL-FLAG300 as an antigen

Fig. 3

A Western blotting to confirm specific immunoreactivity of fusion protein (rGroEL-FLAG300) with an anti-histidine tag antibody. M: pre-stained protein standard marker, 1–8: serial two-fold dilution of purified fusion protein. B serial two-fold dilution of recombinant protein probed with pooled positive melioidosis sera (1–3) and with antibodies from pooled normal sera (4–6)

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