Fig. 3From: Enhanced serodiagnosis of melioidosis by indirect ELISA using the chimeric protein rGroEL-FLAG300 as an antigenA Western blotting to confirm specific immunoreactivity of fusion protein (rGroEL-FLAG300) with an anti-histidine tag antibody. M: pre-stained protein standard marker, 1–8: serial two-fold dilution of purified fusion protein. B serial two-fold dilution of recombinant protein probed with pooled positive melioidosis sera (1–3) and with antibodies from pooled normal sera (4–6)Back to article page