Fig. 2
Detection limit and diagnostics performance of LAMP/CRISPR-Cas12a for identification of N. meningitidis DNA. Left panel: Detection limit of LAMP/CRISPR-Cas12a for identification of N. meningitidis DNA: the fluorescent signals acquired by Bst DNA Polymerase based isothermally amplifying at 55 °C for 30 min on pseudo-samples of 0, 40, 400, 4000, 40,000 and 400,000 and 400,000 copies of N. menitigitidis PCR amplicon spiked into 25 mM Tris–EDTA pH 8 containing the background of 108 copies E. coli PCR amplicon