Fig. 2

Probit analysis of the sigmoid curve that informs the LoD of RT-qPCR SMARTCHEK and RT-qPCR. A RdRP gene for RT-qPCR SMARTCHEK and (B) RdRP gene for RT–qPCR. The X axis shows the expected concentration (copies/reaction). The Y-axis shows a fraction of positive results in all parallel reactions performed. The inner line is a probit curve. Outer lines are the 95% confidence interval (95% CI). The data is representative of three independent experiments with 8 replicates for each concentration