Fig. 1

Graph of the linearity assay to determine the LoD of the RT-qPCR and the RT-qPCR SMARTCHEK. A, B (Control 2019-nCoV_RdRp (ORF1ab)): The expected values (converted to log₁₀) were plotted on the X axis, and the average of the RT-qPCR Ct obtained from the three replicates was plotted on the Y axis (A is for RdRp gene evaluated by RT-qPCR SMARTCHEK and B is for RdRp gene evaluated by RT-qPCR). C, D ( Control 2019-nCoV_E): Expected values (converted to log₁₀) were plotted on the X axis, and the average of the RT-qPCR Ct obtained from the three replicates were plotted on the Y axis (C is for N gene and D is for E gene). Data are representative of three independent experiments with 3 replicates for each concentration. The software used was MedCalc v19.2.1