The impact of nucleic acid testing to detect human immunodeficiency virus, hepatitis C virus, and hepatitis B virus yields from a single blood center in China with 10-years review

Table 3 The results of NAT yields for five different assays using the TMA and PCR methods

Method		TMA				PCR			Overall
Assays		Procleix® Ultrio® Assay	Procleix® Ultrio Plus® Assay	Procleix® Ultrio Elite® Assay	Total	Cobas® TaqScreen MPX Test	Cobas® TaqScreen MPX Test, version 2.0	Total
Number of tested donations		584,540	131,614	444,201	1,160,355	73,052	838,288	911,340	2,071,695
Non-discriminating reactive	Numbers	797	271	695	1763	/	/	/	1763
Non-discriminating reactive	NAT yields (per million)	1363.47	2059.05 **	1564.61 **^##	1519.36	/	/	/	850.99
HBV	Numbers	444	127	708	1279	66	857	923	2202
HBV	NAT yields (per million)	759.57	964.94 *	1593.87 **^##	1102.25	903.47 ^@@	1022.32**^{## @@}	1012.79	1062.90
HCV	Numbers	0	2	0	2	0	0	0	0
HCV	NAT yields (per million)	0.00	15.20 *^@@	0.00	1.72	0.00	0.00	0.00	0.00
HIV	Numbers	1	0	1	2	0	1	1	3
HIV	NAT yields (per million)	1.71	0.00	2.25	1.72	0.00	1.19	1.10	1.45
NAT⁺ELISA⁻ (HBV, HCV and HIV)	Numbers	445	129	709	1283	66	858	924	2207
NAT⁺ELISA⁻ (HBV, HCV and HIV)	NAT yields (per million)	761.28	980.14 *	1596.12 **	1105.70	903.47	1023.51	1013.89 ^&	1065.31
NAT⁺ELISA⁻ (ALL kinds)	Numbers	1242	400	1404	3046	66	858	924	3970
NAT⁺ELISA⁻ (ALL kinds)	NAT yields (per million)	2124.75	3039.19 **	3160.73 **	2625.06	903.47	1023.51	1013.89	1916.31

Bold values indicate significant differences
HBV hepatitis B virus, HCV hepatitis C virus, HIV human immunodeficiency virus, ELISA enzyme linked immunosorbent assay, NAT nucleic acid amplification testing, PCR polymerase chain reaction, TMA transcription-mediated amplification
^*p < 0.05,**p < 0.01, compared to Procleix® Ultrio® Assay; #p < 0.05, ##p < 0.01, compared to Procleix® Ultrio Plus® Assay; @@p < 0.01, compared to Procleix® Ultrio Elite® Assay; &p < 0.05, compared to TMA method

ISSN: 1471-2334