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Table 1 Infections diagnosed by Metagenomic NGs with “Total NA extraction”

From: Comparison and development of a metagenomic next-generation sequencing protocol for combined detection of DNA and RNA pathogens in cerebrospinal fluid

Sample ID

mNGs result with total NA

Clinical etiology

Result

Method

0001

Herpes simplex virus type 1

Herpes simplex virus type 1

Fluorescent quantitative PCR of CSF

0004

Acinetobacter baumannii (0.68)*

Acinetobacter pittii (0.29)

Acinetobacter calcoaceticus(0.18)

Acinetobacter baumannii

Bacterial culture of CSF

0006

Echovirus 30

Enterovirus

Fluorescent quantitative PCR of CSF

0008

Streptococcus agalactiae

Streptococcus agalactiae

Bacterial culture of CSF

0010

Coxsackievirus B5

Enterovirus

Fluorescent quantitative PCR of CSF

  1. *The corresponding genomic coverage of pathogen species is shown in brackets