Fig. 3

Microbiome composition and alpha diversity for RBX2660 and responders before and after treatment. A Sample and group mean (π) relative abundances at the class level for classes comprising at least 3% relative abundance at one or more time points, denoted as before treatment (BL), 7, 30, 60 days after treatment (7D, 30D, 60D), or 6, 12, 24 months after last received RBX2660 treatment (6 M, 12 M, 24 M). Individual samples are represented as dots, and group means (π) with upper and lower confidence intervals (red boxes) were calculated based on maximum likelihood estimate using the Dirichlet multinomial. B Group mean relative abundances (π) with upper and lower confidence intervals for subset of participants who provided all four time points shown—a repeated measurements analysis. C Alpha diversity of RBX2660 and participant samples for each time point group, expressed as the mean and standard deviation of the Shannon indices. Lines with an asterisk (*) indicate statistically significant differences (p < 0.05, t-test) between two time point groups