Effect of heat inactivation for the detection of severe acute respiratory syndrome-corona virus-2 (SARS-CoV-2) with reverse transcription real time polymerase chain reaction (rRT-PCR): evidence from Ethiopian study

Table 3 Analysis of Ct value difference between heat inactivated and non-inactivated Oro-pharyngeal specimen in COVID-19 testing, Ethiopia, 2020

Variables	Mean (95% CI)	Paired T-test differences			Non-parametric Wilcoxon test
Variables	Mean (95% CI)	Mean difference (95% CI)	T-test	p-value	p-value
Inactivated sample ORF1a/b gene Ct	25.4397 (24.369, 26.521)	0.042 (− 0.247, 0.331)	0.288	0.774	0.871
Non-inactivated sample ORF1a/b gene Ct	25.3978 (24.336, 26.438)	0.042 (− 0.247, 0.331)	0.288	0.774	0.871
Inactivated sample N gene Ct	24.1585 (22.925, 25.301)	0.389 (0.097, 0.682)	2.638	0.010	0.000
Non-inactivated sample N gene Ct	23.7689 (22.609, 24.863)	0.389 (0.097, 0.682)	2.638	0.010	0.000
Inactivated sample ORF1a/b gene Ct > 30	34.6269 (33.455, 35.852)	1.262 (0.435, 2.088	3.114	0.004	0.001
Non-inactivated sample ORF1a/b gene Ct > 30	33.3650 (32.427, 34.321)	1.262 (0.435, 2.088	3.114	0.004	0.001
Inactivated sample N gene Ct > 30	33.5155 (32.6796, 34.293)	1.00 (0.439, 1.561)	3.709	0.001	0.000
Non-inactivated sample N gene Ct > 30	32.5155 (31.646, 33.3397)	1.00 (0.439, 1.561)	3.709	0.001	0.000

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