Occurrence and multidrug resistance of Campylobacter spp. at duck farms and associated environmental and anthropogenic risk factors in Bangladesh

Table 1 List of primers and thermal condition used for molecular identification of Campylobacter species

Primer	Sequence (5′-3′)	Target	Amplicon size (bp)	PCR condition (30 cycle)			References
Primer	Sequence (5′-3′)	Target	Amplicon size (bp)	Denature	Annealing	Extension	References
16S9F 16S1540R	GAGTTTGATCCTGGCTC AAGGAGGTGATCCAGCC	16S rRNA	1530	94 °C, 30 s	47 °C, 30 s	72 °C, 90 s	Samosornsuk et al. [21]
HIP400F HIP1134R	GAAGAGGGTTTGGGTGGTG AGCTAGCTTCGCATAATAACTTG	hipO gene	735	94 °C, 30 s	55 °C, 30 s	72 °C, 45 s	Linton et al. [22]
Cj-cdtCU1 Cj-CdtCR2	TTTAGCCTTTGCAACTCCTA AAGGGGTAGCAGCTGTTAA	Cj-cdtC	524	94 °C, 30 s	53 °C, 30 s	72 °C, 30 s	Asakura et al. [23]
Cc-CdtCU1 Cc-CdtCR1	TAGGGATATGCACGCAAAG GCTTAATACAGTTACGATAG	Cc-cdtC	313
CfspCU2 CfspCR1	AAGCATAAGTTTTGCAAACG GTTTGGATTTTCAAATGTTCC	Cf-cdtC	397

ISSN: 1471-2334