Fig. 3

Specificity of the anti-CHIKV human antibodies. Inactivated arbovirus (CHIKV, VEEV, DENV-1, DENV-2, and ZIKV) were coated onto ELISA plates. After the blocking, all the antibodies were used at the same concentration and detected with HRP-conjugated Protein A and enhanced by chemiluminescence (ECL) substrate. For the recognition of each virus, a hyperimmune serum or monoclonal antibodies specific for each virus were used as positive controls (see details in "Method" section). BSA was used as negative control. Corrected OD values are shown. All measurements were caried out in triplicate. Data are shown as average ± standard error of the mean (SEM). Statistical analysis was performed by one-way ANOVA followed by post hoc Tukeys’s multiple comparisons test. *** indicates p < 0.001