Fig. 3

a Multiplex microsphere immunoassay measurement of total antibodies to Zika envelope, Zika NS1, and the NS1 proteins of all four dengue serotypes are presented as the median fluorescence intensity (MFI) of 100 beads counted for each antigen coated beads on the Y axis. The target antigens are reported on the X axis. This multiplex analysis demonstrates past Zika infection. The dramatically lower level of antibodies to dengue NS1 proteins makes a past dengue infection unlikely. b Shows the virus being neutralized. Exceptionally high PRNT to Zika indicates past infection. The lower PRNT to DENV1 and DENV2 may indicate cross reaction of the Zika antibodies recognizing dengue since it is the envelope protein being neutralized