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Table 3 Intra- and inter-assay of 4 pooled sampling

From: Evaluation of pooling of samples for testing SARS-CoV- 2 for mass screening of COVID-19

4 – fold pooled sample

Coefficient of Variation %

Intra assay

 High viral load positive samples

0–2%

 Medium viral load positive samples

0–2%

 Low viral load/ Weak positives

2–141%

Inter assay

 High viral load positive samples

1–2%

 Medium viral load positive samples

1–4%

 Low viral load/ Weak positives

1–173%