Table 1 Data related to antigen concentrations, blocking buffers, Tween 20 percentage and washing numbers during six biopanning rounds
Rounds | I | II | III | IV | V | VI | VII |
|---|---|---|---|---|---|---|---|
Protein (μg/ml) | 375 | 250 | 150 | 120 | 150 | 150 | 150 |
Blocking buffers | %2 skim milk | %3 BSA | %2 skim milk | %3 BSA | %2 skim milk | %3 BSA | %2 skim milk |
% Tween 20 | %0.1 | %0.1 | %0.1 | %0.1 | %0.1 | %0.1 | %0.1 |
Washing numbers | 3 | 20 | 10 | 20 | 20 | 20 | 25 |
Phage incubation time (hrs) | 2 | 2 | 2 | 2 | 2 | 2 | 2 |