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Table 1 Data related to antigen concentrations, blocking buffers, Tween 20 percentage and washing numbers during six biopanning rounds

From: Isolation and characterizations of a novel recombinant scFv antibody against exotoxin A of Pseudomonas aeruginosa

Rounds I II III IV V VI VII
Protein (μg/ml) 375 250 150 120 150 150 150
Blocking buffers %2 skim milk %3 BSA %2 skim milk %3 BSA %2 skim milk %3 BSA %2 skim milk
% Tween 20 %0.1 %0.1 %0.1 %0.1 %0.1 %0.1 %0.1
Washing numbers 3 20 10 20 20 20 25
Phage incubation time (hrs) 2 2 2 2 2 2 2