Fig. 2From: Isolation and characterizations of a novel recombinant scFv antibody against exotoxin A of Pseudomonas aeruginosaThe specificity of the selected phage clones for ExoA-DI. To perform a monoclonal phage ELISA, 60 μg/ml of ExoA-DI was coated per well of ELISA plates, and 100 μl supernatant of each phage clone was separately added per well. Clone number 1 related to the wild-type phage that was used as the negative control. The reactivity was assessed using anti-M13-HRP at 1:2000 dilutions. Absorbance values were recorded as the mean ± SD for three independent determinations. Error bars are indicating the standard deviation for each set of dataBack to article page