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Fig. 2 | BMC Infectious Diseases

Fig. 2

From: Isolation and characterizations of a novel recombinant scFv antibody against exotoxin A of Pseudomonas aeruginosa

Fig. 2

The specificity of the selected phage clones for ExoA-DI. To perform a monoclonal phage ELISA, 60 μg/ml of ExoA-DI was coated per well of ELISA plates, and 100 μl supernatant of each phage clone was separately added per well. Clone number 1 related to the wild-type phage that was used as the negative control. The reactivity was assessed using anti-M13-HRP at 1:2000 dilutions. Absorbance values were recorded as the mean ± SD for three independent determinations. Error bars are indicating the standard deviation for each set of data

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