Table 1 Qualitative assessment of DBS testing; type of extraction, PCR protocol, DBS samples input and overall percentage of DBS punches that tested positive for CMV DNA
From: Diagnosing congenital Cytomegalovirus infection: don’t get rid of dried blood spots
DNA Extraction method | PCR protocol | DBS samples imput | % of CMV-positive DBS with low viral load (< 4 log10 copie/ml) | % of CMV-positive DBS with moderate-high viral load (> 4 log10 copie/ml) | References |
|---|---|---|---|---|---|
QIAamp DNA Investigator Kit (QIAGEN) | Real-time PCR | 3 punches of 3.2 mm | 88% | 97% | [15] |
QIAamp DNA Investigator Kit on QIAcube (QIAGEN) | Real-time PCR | 3 punches of 3.2 mm | 79% | 100% | |
QIAamp DNA Mini Kit (QIAGEN) | Real-time PCR | 3 punches of 3.2 mm | 46% | 91% | |
Thermal shock (methods by Barbi et al.) | Real-time PCR | 1 punches of 6 mm | 100% | 100% | |
Thermal shock (methods by Barbi et al.) | Real-time PCR | 3 punches of 3.2 mm | 60% | 90% | [16] |
KOH-Tris Extracta DBS (QuantaBio) | Real-time PCR | 3 punches of 3.2 mm | 80% | 98% | |
DNA Extract All (Applied Biosystems) | Real-time PCR | 3 punches of 3.2 mm | 83% | 98% | |
Gentra Puregene (QIAGEN) | Real-time PCR | 3 punches of 3.2 mm | 67% | 100% | |
M48 MagAttract DNA Mini ki (QIAGEN) | Real-time PCR | 3 punches of 3.2 mm | 58% | 85% | |
Manual phenol-chloroform method | Conventional PCR | 1 whole Spot (Ø 1 cm) | 66%* | [18] | |
Manual phenol-chloroform method | Real-time PCR | 1 whole Spot (Ø1cm) | 82%* | ||
easyMAG (BioMérieux) | Conventional PCR | 1 whole Spot (Ø 1 cm) | 45%* | ||
easyMAG (BioMerieux) | Real-time PCR | 1 whole Spot (Ø 1 cm) | 73%* | ||