Fig. 5
From: MicroRNA expression profiling of peripheral blood mononuclear cells associated with syphilis
a Detection of PBMCs of different stages miRNAs by the RT-qPCR assay. The expression of eight miRNAs was measured in 106 samples. We analyzed the expression of eight miRNAs (hsa-miR-195-5p, hsa-miR-223-3p, hsa-miR-589-3p, hsa-miR-342-3p, hsa-miR-6511a-3p, hsa-miR-31-5p, hsa-miR-6855-3p) selected from the microarray data by using RT-PCR. Relative expression was used to normalize the relative gene expression data in the RT-qPCR assay. U6 was set as the reference gene. Statistical analysis was performed using the nonparametric Mann-Whitney test. ***P < 0.001, **P < 0.01, *P < 0.05. b PBMCs from healthy individuals were incubated with T. pallidum, and the expression of eight miRNAs was measured by RT-PCR